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A) Expression: SDS-PAGE of bacterial lysate before (Control) and 2h after addition of IPTG (IPTG); B) Purification: SDS-PAGE of purified Trx-Lsd2 (Lane 1); Trx-Lsd2 after thrombin cleavage (Lane 2); Trx-Lsd2 after enterokinase cleavage (Lane 3). C) Scheme of the Trx-Lsd2: The locations of the His-tag (H), S-Tag (S), and the protease cleavage sites for thrombin (T) and enterokinase (E) are indicated.
