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Guanidinium.HCl-induced unfolding was monitored by circular dichroism. CD-spectra were acquired at each concentration of denaturant. The fraction of lost α-helical content was determined from the change in mean residue ellipticity (MRE) at 220nm assuming a value of −3000 deg.cm2/dmol for the MRE corresponding to absence of α-helical content.
