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. 2009 Sep 17;4(9):e7074. doi: 10.1371/journal.pone.0007074

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Fragoso et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunofluorescence analysis of PSSA-2-HA expression by trypanosomes isolated from the midgut, foregut and saliva probes 28–35 days after the infective bloodmeal. Trypanosomes were fixed with formaldehyde and glutaraldehyde and stained with anti-HA antibody (upper panel) and DAPI (lower panel).