Figure 4.

Calcium deposition. The ADSCs were cultured in osteogenic medium in the absence and presence of physiological concentrations of melatonin (0.01-10 nM) for 28 days in flasks (25 cm²) as a monolayer culture. The osteogenic medium alone group was the control group. Calcium depositions were measured as described in Materials and Methods. Values are mean ± SE (*P < 0.05). AD: Adipose-derived stem cells, Cont: control, lM: melatonin 0.01 nM, hM: melatonin 10 nM