Figure 4.

Inflammatory stimuli regulate RGS10 protein localization in primary microglia. A, Single-channel and overlay images of dual-labeling immunofluorescence with the microglial marker CD68 (red) and RGS10 (green) in primary microglia from brains of WT mice after 24 or 48 h LPS (10 ng/ml) treatment in vitro. Insets, High-magnification images of primary microglia. Scale bar, 55 μm. B, Immunoblot analysis of RGS10 protein expression (normalized to α-tubulin) in primary microglia in response to LPS (10 ng/ml) treatment. Veh, Vehicle.