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. Author manuscript; available in PMC: 2009 Sep 8.
Published in final edited form as: Protein Expr Purif. 2006 Feb 13;48(1):151–159. doi: 10.1016/j.pep.2006.01.014

Fig. 6.

Fig. 6

RegIIIγ and HIP/PAP binding to immobilized saccharides. 50 µg of each protein was bound to 25 µl of immobilized mono- or polysaccharide for 2 h at 4 °C. After washing, bound proteins were released by boiling the Sepharose beads in SDS–PAGE sample buffer followed by electrophoresis through 15% Polyacrylamide gels and Coomassie blue staining.