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Kinase activities of AtCRY1 and animal type 1, type 2, and type 4 CRYs. Reaction mixtures in kinase buffer with [γ-32P]ATP label were incubated for 30 min either in dark or under 366 nm light at 2 mW cm−2 for 30 min and analyzed by SDS−PAGE. Left, Coomassie blue stain; right, autoradiogram.
