Figure 1.
Developmental toxicity of genistein on zebrafish embryos. The zebrafish embryos at 24 h post-fertilization were exposed to genistein (1 × 10−4 M, 0.5 × 10−4 M, 0.25 × 10−4 M) or vehicle (ethanol, 0.1%) for 60 h. (A) Heart rates of genistein-treated embryos at 30 h after treatment decreased in dose-dependent manner. (B) Although about 90% of vehicle-treated embryos hatched at 37 h after treatment, genistein treatment decreased the number of hatched embryos. (C) Body lengths of genistein-treated embryos at 37 h after treatment decreased in a dose-dependent manner. (D) At 60 h after genistein treatment, all embryos survived in the vehicle-treated group, but none of the 0.5 × 10−4 M and 1 × 10−4 M genistein-treated embryos survived, and about six embryos survived in the 0.25 × 10−4 M genistein-treated group [genistein concentration: (V) vehicle, (H) 1 × 10−4 M, (M) 0.5 × 10−4 M, (L) 0.25 × 10−4 M]. Data are expressed as mean ± SD. Values of each group with identical letters in each panel were not significantly different (p > 0.05).