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TUNEL assay. To determine whether neural cell damages were related to apoptosis, we conducted a TUNEL assay using the same tissue samples. TUNEL assay results showed apoptotic DNA fragments in (B and D) the brain and (C) spinal cord of 0.25 × 10−4 M genistein-treated zebrafish embryos. (A) vehicle treated zebrafish embryos, (B–D) genistein-treated zebrafish embryos. Bar = 50 μm.
