Table 1.

Metabolite levels changed in the leaves of bass5-2

Compound name	Fold change	P-value	Q-value
Methionine sulfoxide	9.9	0.001	0.05
Methionine	7.5	0.004	0.09
ACC	4.6	0.003	0.08
SMM	3.4	0.003	0.08
SAM	3.3	0.004	0.08
MTA	2.9	0.001	0.06
6-Methylsulfinyl-n-hexyl-glucosinolate	0.4	0.0004	0.04
8-Methylsulfinyl-n-octyl-glucosinolate	0.3	0.00005	0.01
3-Methylsulfinyl-n-propyl-glucosinolate	0.3	0.002	0.08
7-Methylthio-n-heptyl-glucosinolate	0.2	0.003	0.08

Metabolite levels were analyzed in 4–6 replicates. In total, 216 metabolites were detected by means of widely targeted metabolomics (see Supplementary Table S1). Fold change of the average metabolite content in the knockout line compared with that in the wild type was calculated. Metabolites that differentially accumulated in bass5-2 compared with the wild type were identified based on the following criteria: fold change >2 or <0.5, and Q-value (Storey and Tibshirani 2003) of Welch’s t-test < 0.1.