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Cysteine and glutathione suppress rust coloration. Deletion of CYS4 in M22, S288c and YPS163 results in rust coloration, similar to M22, on rich medium supplemented with 1mM copper sulfate. The level of rust coloration is reduced in medium supplemented with 8mM L-cysteine or 2mM L-glutathione. Rust coloration is difficult to see in YPS163 which shows reduced growth in the presence of copper sulfate.
