Table 1.

Rust coloration and drug sensitivity are affected by different set of CYS4 induced genes

		Gene expression relative to M22-CYS4::N123I		Gene deletion phenotype
Gene class	Gene name	M22	M22-CYS4Δ	Rust color	Drug sensitivity
Sulfate transport	SUL1	4.1	16.7*		resistant
	SUL2	1.9	3.1*
	OAC1	1.4*	1.1
Sulfate assimilation	MET3	7.1*	3.3	WW
	ECM17	6.0**	5.1**	WW
	MET10	4.5**	4.5**	WW	resistant
	MET22	3.1*	2.0	W
	MET16	2.4*	1.9*	W
Homocysteine biosynthesis	MET17	4.6**	2.5**	R
	IRC7	1.8**	1.4**		resistant
	MET2	1.6	2.1**
Methionine biosynthesis	MET6	5.1*	2.0
S-AdoMet biosynthesis	SAM1	1.7*	1.2	RR
	SAM2	1.8*	2.0*		resistant
Methionine salvage	ADI1	1.7*	1.0
Cysteine biosynthesis	CYS4	2.5**	0.05**	RR	sensitive
	CYS3	1.5*	1.6*	RR	sensitive
Glutathione biosynthesis	GSH1	1.6	2.1*		sensitive
Siderophore-iron transport	ARN1	1.5**	3.1**
	SIT1	2.9**	2.7**		resistant
	ENB1	2.5*	2.4*
	FIT2	1.6	8.5**		resistant
	FIT3	1.4	7.8**

^**Genes significant at 5% FDR

^*genes significant at 20% FDR

Gene expression ratios are the average of three replicates and the mean squared error is 0.13, averaged across genes. Rust coloration and drug sensitivity phenotypes were measured using strains from the homozygous diploid deletion collection. RR indicates a strong enhancer (< 0.6) and WW a strong suppressor (> 1.3), as measured by the intensity of the green channel from photographs. R indicates a moderate enhancer (< 0.8) and W a moderate suppressor (> 1.2). Drug sensitivity was measured by the growth-delay induced by 2mM atenolol supplemented to rich medium. Strains with less than 4 hours of grown-delay are classified as resistant and those with more than 9 hours of growth-delay are classified as sensitive.