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. Author manuscript; available in PMC: 2009 Sep 8.
Published in final edited form as: J Neurosci Methods. 2007 Jul 4;166(1):53–65. doi: 10.1016/j.jneumeth.2007.06.024

Fig. 4.

Fig. 4

A and B. Influence of age (muscle thickness), blood supply, oxygenated and non-oxygenated Krebs buffer, and method of stimulation on the contractile force of the chicken lateral gastrocnemius muscle. Time course of twitch tension in lateral gastrocnemius muscle elicited (A) via the tibial nerve or (B) by direct muscle stimulation. Animals were examined at post-hatch day 2 (P2) or P21 with blood flow intact (Control) or without blood flow in the presence of non-oxygenated Krebs buffer (No Blood) or oxygenated Krebs buffer (No Blood/O2 Krebs). Note the substantial decrease in force with nerve stimulation after 15–20 min of ischemia. Error bars = SEM. n = 4–7 animals per data point.