. 2009 Jun 16;297(3):E665–E675. doi: 10.1152/ajpendo.00115.2009

Table 3.

Effects of AICAR, contraction, and insulin on AMPK, ACC, and Akt phosphorylation in WT EDL muscle

	pAkt Thr³⁰⁸	pAkt Ser⁴⁷³	pAMPK Thr¹⁷²	pACC Ser²²⁷
Basal	100±8.9	100±11	100±29	100±11
AICAR	148±16^*	174±41^*	266±17^**	520±27^**
Basal	124±26	113±13	101±15	120±21
Insulin	1,989±207^**‡	2,726±215^**‡	98.3±7.6	124±14
Basal	84.0±15	90.1±12	96.8±13	127±23
AICAR + insulin	1,962±168^**	2,641±182^**	279±9.8^**	596±35^**
Basal	115±20	84.8±6.9	125±28	124±19
Contraction	246±29^*	287±23^**	489±32^**§	476±20^**
Basal	133±18	95.8±18	93.4±18	112±11
Insulin + contraction	1,758±159^**	2,413±200^**	486±57^**	469±42^**
Basal	102±14	77.1±11	88.5±14	132±18
AICAR + contraction	274±42^*	336±47^*	454±47^**^a	641±38^**¤

Data are related to basal from AICAR trial and expressed as means ± SE; n = 8. Thr³⁰⁸ and Ser⁴⁷³: increase in phosphorylation was higher in insulin-stimulated muscle than AICAR- or contraction-stimulated muscle (‡P < 0.05). Combining insulin and AICAR or contraction did not increase phosphorylation above insulin levels. pAMPK Thr¹⁷²: contraction increased phosphorylation to a higher extent than AICAR (§P < 0.05); combining AICAR and contraction increased phosphorylation above AICAR (‡P < 0.05) but not contraction levels. pACC Ser²²⁷: AICAR and contraction combined increased phosphorylation to a higher extent than AICAR or contraction alone (^aP < 0.05).

^*/**

P < 0.05/0.01 toward basal.