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. 2009 Jul 1;297(3):F662–F670. doi: 10.1152/ajprenal.00146.2009

Fig. 4.

Fig. 4.

Temporal activation of Raf/MEK/ERK pathway by 5,14-20-HEDE. Western blot analysis was performed to determine early activation of Raf/MEK/ERK pathway after incubation of LLC-PK1 cells with 20 μM 5,14-20-HEDE. Cells were collected 1 min, 5 min, 10 min, 20 min, and 3 h after addition of the drug. Cells were treated with vehicle for 5 min to use as control for comparison with 5,14-20-HEDE-treated cells. Blot was performed twice with samples from 2 different batches of cells studied in triplicate for each time point. β-Actin was used as a loading control.