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. 2009 Jul 1;297(3):F662–F670. doi: 10.1152/ajprenal.00146.2009

Fig. 5.

Fig. 5.

Effect of MEK inhibition on ERK1/2 activation mediated by 20-HETE and 5,14-20-HEDE. Western blot analysis was performed to determine changes in levels of phosphorylated and total forms of MEK1/2 and ERK1/2 after 3 h of incubation with 20 μM 20-HETE (A and B) and 20 μM 5,14-20-HEDE (C and D) in the presence or absence of 10 μM U0126. B and D: densitometry values for 20-HETE and 5,14-20-HEDE. All bands for each respective antibody were run on the same gel. Bands are separated to indicate that the blot was cropped to bring the most relevant grouping of bands together. Blots were performed twice with the lysates from 2 different batches of cells studied in triplicate for each treatment group. β-Actin was used as a loading control in A and C. *Significant difference between groups (P < 0.05).