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. 2009 Jul 1;297(3):F662–F670. doi: 10.1152/ajprenal.00146.2009

Fig. 6.

Fig. 6.

Effect of c-Src and epidermal growth factor receptor (EGFR) inhibition on ERK1/2 activation mediated by 20-HETE and 5,14-20-HEDE. Western blot analysis was performed to determine changes in levels of phosphorylated and total forms of EGFR and ERK1/2 after 3-h incubation with 20 μM 20-HETE (A), EGFR and ERK1/2 after 3-h incubation with 20 μM 5,14-20-HEDE (C), and c-Src and ERK1/2 after 3-h incubation with 20 μM 5,14-20-HEDE (D) in the presence or absence of 0.05 μM SKI-606 or 0.1 μM EKB-569. B and E: band intensities for 20-HETE and 5,14-20-HEDE. β-Actin was used as a loading control in all groups. All bands for each respective antibody were run on the same gel and cropped only to group the relevant bands together. Each blot was performed twice with lysates from at least 2 different experiments with triplicate samples for each treatment group. *Significant difference between groups (P < 0.05).