Fig. 7.

Effect of c-Src inhibition in epidermal growth factor (EGF)-mediated activation of ERK1/2. Representative Western blot analysis for active (phosphorylated) and total EGFR and ERK1/2 protein was performed using LLC-PK₁ cell lysates treated with 0.02 μM EGF (A) or 20 μM 5,14-20-HEDE (C) in the presence or absence of 0.05 μM SKI-606. B and D: band intensities for EGF and 5,14-20-HEDE. β-Actin was used as a loading control. All bands for each respective antibody were run on the same gel and cropped only to group the relevant bands together. *Significant difference between groups (P < 0.05).