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. 2009 Jul 8;297(3):F629–F638. doi: 10.1152/ajprenal.00213.2009

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Copyright © 2009, American Physiological Society

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Fig. 3. — Antigenicity of FLAG-tagged hHO-1. Protein lysates extracted from HeLa cells transfected with pCMV-FLAG-hHO1 or pCMV-FLAG-BAP (bacterial alkaline phosphatase; BAP) were analyzed by Western blotting using an anti-FLAG antibody (top) or anti-HO1 antibody (bottom). The anti-FLAG antibody detected FLAG-tagged hHO-1 (33 kDa; white asterisk) and FLAG-tagged BAP (50 kDa). The anti-HO-1 antibody detected both endogenously expressed HO-1 (32 kDa, 288 amino acids) and FLAG-tagged HO-1 (33 kDa, 301 amino acids).