Fig. 4.

Summary of characteristics of mice that originated from one of the founders (founder 6). In the table, F2 hemizygotes from founder 6 generated 23 F3 pups, shown as 3 separate litter groups based on their dates of birth (DOB). The table summarizes gender, weight, genotype assessed by Southern blot analysis and PCR (+ or − indicates the presence or absence of the FLAG-hHO-1 transgene), and urine albumin (Ualb) excretion factored by that of urine creatinine (Uc). Zygosity was determined using real-time quantitative PCR (Q-PCR) and 2^−ΔΔCt method (described in materials and methods).