Fig. 5.

Expression of fibronectin (FN) and collagen IV. A: RT-PCR determination of FN and collagen IV (Col-4) mRNA expression in the kidney of all treatment groups as indicated. B2M, β-2 microglobulin. B: densitometric quantification of FN and Col-4 mRNA levels based on the RT-PCR data. C: glomerular immunostaining of FN. Kidney sections from nondiabetic control (C) and diabetic mice treated with V, L, DL, L+DL, or L+DH were immunostained with anti-FN antibody. *P < 0.05, **P < 0.01 vs. V. ##P < 0.01 vs. C; n = 6–7.