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Expression and in-gel kinase assay of NH2-terminal truncated WNK4 protein. A: FL and the indicated truncated WNK4 proteins were expressed and recovered from HEK293 cells with the regular TAP protocol and then separated on a 7.5% polyacrylamide gel and visualized by silver staining. B: assay of in-gel phosphorylation of OSR1-D164A by the NH2-terminal truncated WNK4 594–1222, compared with FL WNK4 and Δ593. 32P-phosphorylated proteins were visualized by 3-h autoradiography.
