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. Author manuscript; available in PMC: 2010 May 17.
Published in final edited form as: Aquat Toxicol. 2009 Mar 9;92(4):281–287. doi: 10.1016/j.aquatox.2009.02.017

Figure 3.

Figure 3

Effect of time (A) and substrate concentration (B) on the conversion of BDE 99 to BDE 49. In panel (A) the conversion of BDE 99 to BDE 49 occurred a 2 ρmol/hour/mg protein, linearly until 16 hours in Chinook microsomal fractions incubated with 0.9 μM BDE 99. The heat-inactivated controls did not contain detectable levels of BDE 49. In panel (B), we did not observe substrate saturation with increasing concentrations of BDE 99 (from 0.03 μM to 1.7 μM) over 24 hours. In both panels, the assays were conducted using Chinook salmon microsomes from an individual liver, and error bars represent mean ±SD for triplicate assays (n=3) and indicate technical variation.