Table 1.
Comparative debromination of BDE 99 to tetrabrominated congeners by Chinook salmon and common carp microsomal fractions.
| Percent Congener Formed (±SD)b |
|||
|---|---|---|---|
| Species | Incubation conditionsa | BDE 47 | BDE 49 |
| Chinook salmon |
+ NADPH (25 °C) | BDL | 2.1 (±0.2)% |
| − NADPHc (25 °C) | BDL | 2.7(±0.04)% | |
| −NADPH, − DTTc (25 °C) | BDL | BDL | |
| + NADPH (15 °C) | BDL | 1.4 (±0.1)% | |
| Common Carp |
+ NADPH (25 °C) | 53 (±7)% | BDL |
a
Chinook liver microsomes and carp liver microsomes were incubated with 0.9 μM BDE 99 and 10 mM DTT (except where noted) as described in materials and methods
b
Data represent mean ± standard deviation of technical triplicates. BDL= below detection limit. Percent recovery of surrogate was 87±8%.
c
Chinook microsomes for these incubations were prepared without DTT in the homogenenization, wash or resuspension buffers.