Table 2.
Comparative debromination of BDE 99 to tetrabrominated congeners by Chinook and carp cytosolic fractions.
| Amount of BDE 47 formed | Amount of BDE 49 formed | ||||
|---|---|---|---|---|---|
| Species | Cytosol incubation conditionsa |
Mean ρmols |
% formation (±SD) |
Mean ρmols |
% formation (±SD) |
| Chinook salmon |
Undialyzed + 5mM GSH |
BDL | n/a | 2.8 | 0.3 (±0.09)% |
| Undialyzed, no added GSH |
BDL | n/a | 2.5 | 0.3 (±0.03)% | |
| Dialyzed + 5mM GSH |
BDL | n/a | 1.7 | 0.2 (±0.03)% | |
| Dialyzed, no added GSH |
BDL | n/a | 1.7 | 0.2 (±0.03)% | |
| Common carpb |
Undialyzed + 5mM GSH |
561 | 62 (±2.7)% | 4.1 | 0.5 (±0.04)% |
| Undialyzed, no added GSH |
524 | 58 (±2.7)% | 4.1 | 0.5 (±0.08)% | |
| Dialyzed + 5mM GSH |
472 | 53 (±2.4)% | 3.9 | 0.4 (±0.05)% | |
| Dialyzed, no added GSH |
495 | 56 (±2.7)% | 3.8 | 0.4 (±0.03)% | |
a
Reactions contained a initial substrate concentration of 0.9 μM BDE 99 and were incubated for 16 hours.
b
Carp data has been surrogate recovery corrected because surrogate recovery in carp cytosolic experiment ranged from 60.5-101.5%.