Figure 1. Regional and Neocortical Areal Gene Expression Profiling in the Mid-Fetal Human Brain.
(A) Human late mid-fetal brain illustrating locations of tissue samples microdissected from nine areas of neocortex (NCTX; including four areas of the prefrontal cortex [PFC]), hippocampus (HIP), striatum (STR), mediodorsal thalamus (THM), and cerebellum (CBL) from both sides of four mid-fetal brains. Dashed lines (i, ii) indicate levels of acetylcholine esterase-reacted coronal tissue sections.
(B) Comparison among NCTX, HIP, STR, THM, and CBL detected 76% of core genes expressed above background in at least one brain region. At a FDR of 10−5, 33% of these were DEX and 28% DAS between regions.
(C) Intra-NCTX analysis yielded fewer DEX and DAS genes, even at a relaxed threshold (FDR = 0.01).