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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Anal Chem. 2009 May 15;81(10):4060–4067. doi: 10.1021/ac900265f

Figure 2.

Figure 2

Electroporation of a target A549 cells. (A) Bright field image of the capillary and A549 cells in a cell dish before electroporation. A 2-µm opening capillary tip filled with extracellular buffer/pEGFP-C2 plasmid is placed from the left of the target cell with a dm of 5 µm. (B) Thioglo 1-GSH adduct fluorescence image of the cells before the pulse. (C) Thioglo 1-GSH adduct fluorescence image of the cells 40 s after the pulse. (D) The target cell Thioglo 1-GSH adduct fluorescence decay curve. Fluorescence intensity drops suddenly when the pulse is trigged followed by a slow decay while the transient pores real. (All images taken using a 20× objective, scale bar 20 µm)