Table 1.
Effect of pulse types on SCEP induced pEGFP-C2 transfection
Electroporated Cells ΔF% | Pulse type |
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---|---|---|---|---|---|---|---|---|---|---|
300 ms |
2*150 ms with 150 ms interval |
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Total # | Missing€ # | Missing % | Transfected # | Transfected % | Total # | Missing€ # | Missing % | Transfected # | Transfected % | |
0–10% | 5 | 0 | 0.0 | 0 | 0.0 | 10 | 1 | 10.0 | 0 | 0.0 |
10–40% | 8 | 4 | 50.0 | 5 | 50.0 | 20 | 1 | 5.0 | 19 | 95.0 |
40–80% | 11 | 7 | 63.6 | 4 | 36.4 | 8 | 2 | 25.0 | 6 | 75.0 |
Total | 24 | 11 | 45.8 | 8 | 33.3 | 38 | 4 | 10.5 | 25 | 65.8 |
Missing cells 24 hours after electroporation