TABLE 3.

Recovery of MA from human serum

Sample (X0 + na) (n = 0,1,2,3)	Amount Added	Amount Found			Recoveryb (Mean ± SD)	Estimated Amount ± 95% Confidence Limitc
	pmol				%	pmol
X0	0	$\overline{X}$0 ± SD = 15.0 ± 0.6a				14.1 ± 1.1
X0 + a	9.6	23.6	25.2	25.0
X0 + a	9.6	24.2	23.6	23.3	94.5 ± 8.2
X0 + 2a	19.2	33.2	32.1	32.0
X0 + 2a	19.2	33.9	34.1	34.0	94.6 ± 5.1
X0 + 3a	28.8	43.8	43.9	43.0
X0 + 3a	28.8	44.1	43.6	43.2	99.0 ± 1.5

Known amounts of MA were spiked into 50 μl of normal human serum before sample preparation.

^aThe value was obtained from Table 1.

^bRecovery (%) = (amount found – $\overline{X}$₀) / amount added × 100.

^cThe estimated amount was calculated by orthogonal regression.