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. 2009 Oct;50(10):2014–2026. doi: 10.1194/jlr.M800659-JLR200

Fig. 2.

Fig. 2.

Accumulation of lipids in THP-1 macrophages incubated with TG-DISP and/or CE-DISP. THP-1 macrophages were treated for 48 h at 37°C in RPMI containing 1% FBS and TPA (50 ng/ml) alone or with the specified concentrations of lipid dispersions. Cells were cultured using the inverted cell culture technique as described in Materials and Methods. A: Incubation of THP-1 with 50 μg TG/ml TG dispersions (TG-DISP) alone did not significantly (P < 0.05) alter cellular FC (dark-gray portion of bar) or CE (light-gray portion of bar). In contrast, incubation of cells with 60 μg CE/ml CE dispersions (CE-DISP) significantly increased the CE stores in cells. Coincubation of cells with both CE-DISP and TG-DISP produced a significant (P < 0.05) decrease in CE accumulation. B: Incubation of THP-1 with 50 μg TG/ml TG-DISP either alone or in combination with 60 μg CE/ml CE-DISP produced a significant (P < 0.05) increase in cellular TG compared with control cells or cells treated with CE-DISP alone. Values are the mean ± SEM for three experiments. Within each panel, bars with the same letter indicate that means were not statistically different. All other comparisons were significantly different (P < 0.05).