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Ionomycin (Io) increases mouse sGC-α1 promoter activity via NFATc. Rat aortic smooth muscle cells (RAoSMC; A) and human pulmonary artery smooth muscle cells (HPASMC; B) were cotransfected with sGC-luc and Renilla-luc plasmids and treated with 1 μM Io for 24 h ± 1 μM CsA. Values are means ± SE; n = 7. Dual-luciferase activity was measured and expressed as %change of relative luciferase units/relative Renilla units (RLU/ RRU) from basal. Values are means ± SE; n = 6. *P < 0.05 vs. basal.
