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Other muscle genes. The mRNA expression of creatine kinase and myostatin is unchanged during mechanical overload, but hypoxia-inducible factor-1α (HIF-1α) is strongly upregulated (n = 13, *P = 0.006). This may reflect the metabolic strain on the plantaris from increased mechanical loading and its relative inability to enhance oxidative mechanisms. Data are means ± SE; expression was normalized to ribosomal 18s.
