. 2009 Jun 24;297(3):R578–R586. doi: 10.1152/ajpregu.00236.2009

Table 1.

Primers used in reverse-transcriptase PCR

	5′ Forward Primer	3′ Reverse Primer
Alt 18S	`AGGAATTGACGGAAGGGCAC`	`GTGCAGCCCCGGACATCTAAG`
Alt 18S competimer	`GAATTGACGGAAGGGCACTT`	`GCAGCCCCGGACATCTAAGAA`
Classic 18S	`TCAAGAACGAAAGTCGGAGG`	`GGACATCTAAGGGCATCACA`
Classic 18S competimer	`TCAAGAACGAAAGTCGGAGGA`	`GGACATCTAAGGGCATCACAT`
Creatine kinase	`GAGCACCTGGGCTACGTG`	`TCCAGCTTCTTCTCCATCTCCA`
FOXO-1a	`AAATGATGAATCCCAGCTCCCA`	`TTGGTTGGGCAACACATTGTCAA`
FOXO-3	`CAGTCGGACCCCTTGATGT`	`GCGCCCTGGGTTTGGTG`
HIF-1α	`TTTTAATACCCTCTGATTTAGCATGT`	`AGCTCTGAGTAATTCTTCACCC`
IGF-BP5	`GAGAAGCCGCTGCACGC`	`GGGTCAGCTTCTTTCTGCGA`
IGF1	`GCTCTTCAGTTCGTGTGTGG`	`GTCTTGGGCATGTCAGTGTG`
MAFbx	`AGACCGGCTACTGTGGAAGAG`	`CCGTGCATGGATGGTCAGTG`
MURF	`GCCATCCTGGACGAGAAGAA`	`CAGCTGGCAGCCCTTGGA`
Myostatin	`CAAATCCTGAGACTCATCAAACC`	`ATTTCAATCCTAAGTTGGATTCAGG`
S. lateralis MHC1	`AGAAGGAGCAGGACACCAGC`	`CGCCAATGTCACGGCTCTTG`
S. lateralis MHC2b	`As above`	`CTCTGCAGATTTTATTTCACTGATATAC`
S. lateralis MHC2x	`As above`	`CCAAAAGTAATAAGTACAAAACAGAGTG`

S. lateralis, Spermophilus lateralis; FOXO, transcription factor; HIF, hypoxia-inducible factor-1α; MAFbx and MURF, ubiquitin ligases; MHC, myosin heavy-chain.