Fig. 2.

Effect of obesity on glutamine transport and B⁰AT1 expression. A: brush border membrane glutamine transport activity. [³H]glutamine (50 μM) transport activity was measured in brush border membrane vesicles isolated from biliopancreatic limb, Roux limb, and common channel from lean (Lean, n = 8) and obese Zucker rats (Obese, n = 12) as described in materials and methods. Data are means ± SE. *P < 0.05 by t-test. B: brush border membrane glutamine transporter B⁰AT1 protein levels. B⁰AT1 protein levels were measured in brush border membrane isolated from biliopancreatic limb, Roux limb, and common channel from lean (n = 8) and obese Zucker rats (n = 8) by Western blot as described in materials and methods. Data are means ± SE. *P < 0.05. C: brush border membrane glutamine transporter B⁰AT1 mRNA levels. B⁰AT1 mRNA levels were measured in whole gut segments isolated from biliopancreatic limb, Roux limb, and common channel from lean (n = 8) and Obese Zucker rats (n = 8) by standard Northern blot as described in materials and methods. Data are means ± SE. *P < 0.05.