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. 2009 Jul 16;297(3):G442–G450. doi: 10.1152/ajpgi.00182.2009

Fig. 3.

Fig. 3.

Evolution of mRNA expression levels of TLRs from the ileum of formula-fed and formula-fed-hypoxic rats over 72 h. RNA was isolated from the ileum of newborn rats subjected to formula feeding alone and formula feeding in combination with hypoxia for 24, 48 and 72 h, respectively. Quantitative RT-PCR (qRT-PCR) analysis was performed to determine the expression level (fold changes) of TLRs comparing formula-fed-hypoxic rats with formula-fed rats. Data are represented as means ± SE from 3 independent experiments. The cutoff values of fold change of formula-fed-hypoxic compared with the formula-fed group: > +2 indicating upregulation, < −2 downregulation. Fold changes at 48 and 72 h compared with those at 24 h: *P < 0.01, **P < 0.001. Fold change at 72 h compared with those at 48 h: #P < 0.01, ##P < 0.001. All mRNA expression levels of TLRs differed significantly at 48 h, when formula-fed-hypoxic rats were compared with formula-fed rats.