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. Author manuscript; available in PMC: 2009 Sep 8.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Jan 10;50(5):2237–2244. doi: 10.1167/iovs.08-2785

Figure 3.

Figure 3

Traversal of multilayered corneal epithelial cells grown on semipermeable filters in vitro by P. aeruginosa strain PAK and its twitching motility mutants (pilA, pilU, and pilT) after inoculation of the upper chamber with 106 CFU bacteria. (A) Each of the three twitching motility mutants (pilA, pilU, and pilT) was defective in its ability to traverse cells over 6 hours compared with wild-type PAK (P < 0.05, t-test). (B) Neither wild-type nor twitching motility mutants impacted TER during the 6-hour assay. EGTA-treated samples significantly reduced TER to baseline values (*P < 0.05, t-test, vs. untreated samples at each time point). (C) Growth rates of the twitching mutants were similar to wild-type PAK, as shown by the number of bacteria in the upper chamber over the 6-hour assay. Data are expressed as the mean ± SD. Three semipermeable filters were used for each sample.