Table 1.
Mean (±SD) TER Reading (Ω/cm2) at Postinoculation Time Points |
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0 h | 1 h | 2 h | 3 h | 4 h | 5 h | 6 h | 7 h | 8 h | |
Wild-type PAK* | 135 ± 1 | 132 ± 2 | 131 ± 2 | 130 ± 2 | 130 ± 2 | 127 ± 2 | 125 ± 1 | 123 ± 2 | 121 ± 2 |
No bacteria control | 133 ± 2 | 132 ± 2 | 131 ± 3 | 130 ± 2 | 129 ± 2 | 128 ± 3 | 128 ± 3 | 124 ± 2 | 123 ± 2 |
EGTA control† | 104 ± 2 | 100 ± 2 | 99 ± 3 | 100 ± 1 | 99 ± 1 | 99 ± 1 | 99 ± 1 | 92 ± 2 | 97 ± 2 |
Layers of infected cells showed TER values similar to those of uninfected (HBSS-inoculated) cells throughout the 8-hour traversal assay. Each remained significantly higher than in controls treated with 100 mM EGTA (P < 0.05, t-test).
No significant difference versus uninfected cells at each time point (P < 0.05, t-test).
Similar to baseline control (submerged filter without epithelial cells).