Figure 4.

Reduced expression of mitotic checkpoint genes in chronic HCV patients. a & c) Western blotting for MAD2L1 and KNTC1 was done in total protein extracts prepared from biopsy specimens of chronic hepatitis C or Non-viral hepatitis patients (NVH). Following SDS-PAGE (12% gel) and transfer, the nitrocellulose membranes were probed with monoclonal antibodies to MAD2L1, which recognizes both phosphorylated and de-phosphorylated forms and KNTC1. b & d) Densitometry analysis of total MAD2L1 or KNTC1 expression normalized to their respective loading controls (β-actin) was done using Image J software. The number of samples analyzed in each group (n) is indicated below each column. The p-values at the top of the column compare early HCV to advanced HCV. Represented are mean ± S.D.