Table 3.
List of differentially expressed cell cycle genes >2 fold in advanced HCV relative to early HCV
| Gene symbol | Average Ct Adv. HCV | Average Ct Early HCV | T-test p-value |
Fold change |
|---|---|---|---|---|
| Cell proliferation genes | ||||
| CCND1 | 32.65 | 31.00 | 0.14 | -2.22 |
| CCND2 | 31.3 | 32.35 | 0.05 | 5.00 |
| CDK2 | 32.1 | 30.3 | 0.6 | -2.46 |
| CDK inhibitors | ||||
| CDKN2B | 32.4 | 33.50 | 0.04 | 3.03 |
| CDKN3 | 34.20 | 32.73 | 0.05 | 5.46 |
| CDKN1B | 29.4 | 27.7 | 0.5 | -2.3 |
| G2/M checkpoint gene | ||||
| KPNA2 | 30.85 | 31.3 | 0.98 | 2.75 |
| HERC5 | 33.05 | 29.3 | 0.217 | -8.70 |
| DNA damage response genes | ||||
| ATR | 31.9 | 35 | 0.30 | 10.50 |
| RAD1 | 30.4 | 32.8 | 0.05 | 6.46 |
| HUS1 | 27.55 | 29.65 | 0.05 | 6.06 |
| RAD17 | 28.45 | 30.35 | 0.057 | 5.27 |
| RPA3 | 33.3 | 35.0 | 0.01 | 4.6 |
| *TP53 | 30.80 | 27.03 | 0.04 | -2.79 |
| Mitotic checkpoint regulators | ||||
| MAD2L1 | 35 | 32 | 0.04 | -5.6 |
| CDC34 | 32.65 | 30.00 | 0.025 | -4.4 |
| Cell cycle regulation | ||||
| CCNH | 32.85 | 35.0 | 0.5 | 5.00 |
| CDK8 | 28.7 | 30.5 | 0.07 | 4.92 |
| CCNG2 | 27.8 | 29.85 | 0.09 | 5.86 |
| CKS2 | 30.7 | 32.15 | 0.23 | 3.86 |
| CUL1 | 30.6 | 28.3 | 0.65 | -3.48 |
| TFDP2 | 28.2 | 30 | 0.08 | 4.92 |
| CCNT1 | 28.6 | 31.15 | 0.08 | 4.59 |
The values present average Ct values without normalization and fold differences observed in Real time PCR and p-values of T-test (values ≤ 0.05 are in bold).