Skip to main content
. 2009 Sep 8;106(38):16351–16356. doi: 10.1073/pnas.0906922106

Fig. 3.

Fig. 3.

XMRV proteins are expressed primarily in malignant epithelial cells and very rarely in stromal cells. (A and B) IHC of a section from a qPCR-positive prostate cancer (A) and its diagrammatic representation (B). Nuclei of malignant cells are large and contain ≥1 large nucleoli (B). Multiple acini of malignant epithelial cells (E+) stain positive. All cells within these acini show intense staining. The stroma (S) and a few other acini (E−) are unstained. Insets (A1 and B1) show corresponding fields at higher magnification, with granular cytoplasmic staining pattern in several malignant epithelial cells. (C) A different field from the same sample as in A shows the range of XMRV protein expression in various acini: fewer cells expressing less protein but the same granular staining pattern. (D–F) Three additional representative samples with different frequencies of malignant epithelial cell clusters and different extents of XMRV protein expression. The intracellular staining pattern remains granular in all. (G) Staining limited to part of the cytoplasm of malignant epithelial cells in a subset of samples, as in this sample from which the XMRV clone VP62 was isolated, courtesy of R. H. Silverman and C. Magi-Galluzzi, Cleveland Clinic (6). (H and I) Scattered rare stromal cells showing cytoplasmic staining were seen close to malignant cells (H) or within inflammatory infiltrates (I).