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(A) Schematic of FBP and its bioluminescence reaction. (B) SDS/PAGE of biotinylated CLuc (lane 1) and FBP (lane 2). (C) Bioluminescence spectra of FBP measured in different pH buffer solutions containing 0.1 M NaCl. Curves all superimpose. (D) Bioluminescence spectrum of FBP in blood. (E) Relative light intensity of FBP or CLuc in buffer. (F) Relative light intensity of FBP or CLuc in blood.
