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Antibodies linked to avidin. (A) Purification of avidin-bound antibody by gel filtration column chromatography on TSK gel G3000SW column. (B) Bioluminescence ELISA was used to detect antibody-avidin conjugates; asterisk represents collected fraction. (C) Purification of reduced antibody-avidin by gel filtration column chromatography. (D) Bioluminescent ELISA was used to detect reduced antibody-avidin conjugates; asterisk represents collected fraction. RLU, relative luminescence unit.
