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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Adv Appl Microbiol. 2009;68:133–216. doi: 10.1016/S0065-2164(09)01204-0

Figure 1.

Figure 1

Different micrographs and compositions showing the polysaccharide capsule of C. neoformans. A) Suspension of the cells in India Ink; B) Scanning electron microscopy; C-H) Immunofluorescence using specific mAbs to the capsule (green and red fluorescence) showing also the cell wall localization (blue flurorescence). D) 3D image composition of a C. neoformans cell labeled with two different mAbs to the capsule. In blue, the cell wall. E) Side view of a section of cell shown in D. F-H) Sections showing the 3 dimension of the capsule, visualized after staining with mAbs (green and red). Pictures by Oscar Zaragoza, and from (Maxson et al, 2007b).