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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cancer Lett. 2009 May 12;284(2):157–164. doi: 10.1016/j.canlet.2009.04.016

Fig. 4.

Fig. 4

Ectopic expression of PF increases cell sensitivity to camptothecin. (A) Verification of PF expression by Western blot analysis. Whole cell lysate of RD/Vector or RD/PF cells were analyzed by Western blotting with FKHR-specific antibody and anti β-actin antibody (as loading control). (B) Cell proliferation assay. RD/Vector or RD/PF cells were treated with 0.1% DMSO or 150 nM camptothecin for 48 h. Cell viability was determined by the CellTiter-Glo® assay. Data represent 3 independent experiments, each performed in triplicate. Error bars indicate the standard error of the mean. Difference between the 2 cell lines was significant (p < 0.001) (***).