Skip to main content
. 2009 Aug 31;41(8):577–583. doi: 10.3858/emm.2009.41.8.063

Figure 4.

Figure 4

Adiponectin and rosiglitazone regulate hepatic glucose production. (A) Effects of adiponectin on CRTC2-dependent regulation of CRE activity in primary hepatocytes (**P < 0.01, n = 3 each). Adenoviruses for adiponectin, WT or S171A CRTC2 and CRE-luc were infected for 16 h and then cultured for additional 24 h with 10 µM forskolin (FSK) treatment for 4 h (for luciferase assay) before being harvested. (B) Effects of adiponectin (top) and rosiglitazone (bottom) on glucose production in primary hepatocytes (*P < 0.05, **P < 0.01, n = 3 each). Glucose output assay was performed using primary hepatocytes with 16 h-infection of Ad adiponectin or 12 h-treatment of 50-100 µM rosiglitazone in the absence or in the presence of 10 µM forskolin (FSK). (C) Effects of Ad-adiponectin on hepatic Ad-CRE luc activity. In vivo luminescence was measured 5 days post adenovirus infection as described in methods. Graphic representation of ROI (left) and its quantitation (right) shown (**P < 0.01, n = 3 each). Data in A-C represent mean ± standard deviation from at least three independent experiments.