FIGURE 3. Activation and homeostasis of p105-deficient CD4 T cells.

A, WT, nfκb1^−/−, and p105^−/− naïve CD4 T cells were either not treated (NT) or stimulated in triplicate wells with the indicated amounts of plate-bound anti-CD3 plus soluble anti-CD28. After 48 hr, cell proliferation was measured by thymidine incorporation. Data are presented as mean ± s.d. of three replicates. B, CD4 T cells were either not treated (NT) or stimulated for 24 h with anti-CD3 and anti-CD28. Nuclear extracts were subjected to EMSA using a ³²P-radiolabeled κB probe (upper) or a probe that binds the constitutive nuclear protein nuclear factor Y (NF-Y). C, Mesenteric lymph node cells and splenocytes of the indicated mice were subjected to flow cytometry analyses to measure the percentage of naïve (CD44^hiCD62L^lo) and memory/effector (CD44^loCD62L^hi) CD4 T cells within the CD4 T-cell population.