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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):3131–3138. doi: 10.4049/jimmunol.0803637

FIGURE 3. Activation and homeostasis of p105-deficient CD4 T cells.

FIGURE 3

A, WT, nfκb1−/−, and p105−/− naïve CD4 T cells were either not treated (NT) or stimulated in triplicate wells with the indicated amounts of plate-bound anti-CD3 plus soluble anti-CD28. After 48 hr, cell proliferation was measured by thymidine incorporation. Data are presented as mean ± s.d. of three replicates. B, CD4 T cells were either not treated (NT) or stimulated for 24 h with anti-CD3 and anti-CD28. Nuclear extracts were subjected to EMSA using a 32P-radiolabeled κB probe (upper) or a probe that binds the constitutive nuclear protein nuclear factor Y (NF-Y). C, Mesenteric lymph node cells and splenocytes of the indicated mice were subjected to flow cytometry analyses to measure the percentage of naïve (CD44hiCD62Llo) and memory/effector (CD44loCD62Lhi) CD4 T cells within the CD4 T-cell population.