FIGURE 5. Loss of p105 causes aberrant production of Th17 cells.

A, Th17 frequency in total T cells. T cells were isolated from the spleen of WT or p105^−/− mice and either left non-treated (NT) or stimulated with PMA (50 ng/ml) plus ionomycin (500 ng/ml) for 5 h. The IL-17 producing Th17 cells and the IFN-γ producing Th1 cells were quantified by intracellular cytokine staining and flow cytometry. Numbers represent percentage of CD4⁺ gated cells. B, Th17 frequency in sorted memory CD4⁺ T cells (CD62L^low, CD44^hi, CD25⁻, CD4⁺), determined as in a. C, Expression of Th17 marker genes in the colons of p105^−/− mice. Real-time PCR was performed to analyze the relative RNA concentration of the indicated genes in the colons of WT and p105^−/− mice.