Figure 4. Inverse correlation between miR-126 and p85β in primary colon tumors.
The upper panel shows the qRT-PCR quantification of miR-126 and lower panel shows the western blot analysis of p85β protein levels in matched colon normal (N) and primary colon tumor (T) pairs. miR-126 in tumors is expressed as fold change relative to their matched normals. Note the significant downregulation of miR-126 (≥2-fold, t-test p<0.05) and overexpression of p85β protein in each of the colon tumors. 5S-rRNA and β–actin protein were used as normalization controls for miR-126 and p85β protein, respectively.