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. Author manuscript; available in PMC: 2010 Jul 1.

Published in final edited form as: J Microbiol Methods. 2009 Apr 23;78(1):97–100. doi: 10.1016/j.mimet.2009.04.007

Fig 1 — (A) The mRG1−1 cells were seeded on 16-well E-plates at the 0 hr time point. After an overnight culture, the cells were exposed to the indicated amount of toxins in the absence or presence of A1H3 (H3). The control groups included the cells with PBS, rabbit antiserum, or A1H3 alone. (B) The freshly thawed mRG1−1 cells were seeded on 16-well E-plates simultaneously with a mixture of the same amount of TcdA and TcdB in the absence or presence of A1H3 (H3). In the serum blocking experiment, the rabbit-anti-TcdA serum (pAb) or goat anti-TcdA and –TcdB serum (polyAb) was mixed with the toxins and A1H3 and then added to the cells. The dynamic changes in CI were recorded by RT-CES at a 15-min interval. The data shown here is from the representative experiments.

Fig 1 — (A) The mRG1−1 cells were seeded on 16-well E-plates at the 0 hr time point. After an overnight culture, the cells were exposed to the indicated amount of toxins in the absence or presence of A1H3 (H3). The control groups included the cells with PBS, rabbit antiserum, or A1H3 alone. (B) The freshly thawed mRG1−1 cells were seeded on 16-well E-plates simultaneously with a mixture of the same amount of TcdA and TcdB in the absence or presence of A1H3 (H3). In the serum blocking experiment, the rabbit-anti-TcdA serum (pAb) or goat anti-TcdA and –TcdB serum (polyAb) was mixed with the toxins and A1H3 and then added to the cells. The dynamic changes in CI were recorded by RT-CES at a 15-min interval. The data shown here is from the representative experiments.